TotiCell
Micro and small volume Bioreactor
It can also support three (3) types of images, such as optical images, confocal images, and hologram images. In addition users have the choice to select the ideal one considering the prices and characteristics of the live cell image devices. If images are transmitted to a computer, the image analysis software in the computer can perform researcher-specified patterns based on specific cell counting as well as general cell counting. In addition, it provides the solution that can trace and analyze the change that occurs during of live cell imagery, cell number and tracking including chemotaxis in accordance with manipulation during cell culture. Cell culture vessels are provided for suspension cells and cell culture cartridges for adherent cells. Each having a variety types of vessels or cartridges designed to fit various types of cell characteristics. Our policy is to provide users with optimal customized cell culture and cell culture cartridges according to the customers’special requests.
Cell culture vessels and cell culture cartridges have a separate independent temperature control device, which can perform a precise temperature control. Also, the temperature of the inside of the bio safety chamber can be controlled accurately, assisting in the direct control of the temperature in the cell culture vessel and cell culture cartridge, thus providing the best environment for maintaining precise temperature. As there are 4 gas supplying ports for O2,CO2,N2, and air inside the bio safety chamber, it can directly infuse gas quantities into the cell culture cartridge by specific amounts, for example, 0.01 ~ 21% O2, or 0 ~ 10% CO2, in explicit intervals, and also can install an independent mixed vessel in the front end and dissolve several gases, and then infuse them one at a time.
Also, some researchers want to provide the cultured cell with outside O2, CO2 gases by mounting a gas permeable membrane on the cell culture cartridge. For this purpose, the bio safety chamber inside has the ability to supply O2 and CO2 gases and maintain a suitable level.
The small and sophisticated bio safety chamber that is able to completely prevent contamination from outside can be adjusted for biohazard cleanliness levels by users from class 100 to class 100,000 for the necessary aseptic work area, and we are well prepared for that with a variety of HEPA filters and circulation fans .For the optimized culture of cells used for stem cell therapy as well as cell therapy, a variety types of cell culture cartridges that fit with the culture characteristic of each cell have been prepared. Cell culture cartridges are mainly designed for adherent cells, whereas, suspension cells are mainly used in glass vessels.
We provide customers with optimal customized cell culture cartridges taking into consideration the type and amount of supplying substances that are suitable for the cell to be cultured, such as cell culture capacity, cytokine, and nutrient, membrane type and structural arrangement, as well as type and optimal capacity of gas to be supplied to the cell.
Although it is the same cell, the design might be different depending on the research purpose of mass culture and differentiation of study. For example, even if it is the same cell, the results may be different between the suspension culture and adherent cell culture. As there are various types of material and structure in micro carrier or scaffold, soft agar gel, the culture results can be different every time. Furthermore if vital stimuli are applied, the results can also vary, so the bioreactor design should be made to fit each individual research need. However, there has been no bioreactor that could satisfy the variety of conditions of researchers‘ needs. Therefore cell therapy research can not help staying at this limited boundary. The cell therapy research through those varieties of bioreactor comparison research can produce momentous results in both academic and commercial areas.
Our policy is to provide researchers with optimal designed cartridges and vessels in accordance with whatever kinds of requests the researcher proposes.
For stem cell media, generally like wise conventional animal cell culture, some suitable amount of FBS(or, serum-free) and antibiotics are added to basal medium, such as DMEM, IMDM, MEMa, and RPMI 1640.
For example, for hematopoietic stem cell culture, several cytokines such as IL-3, IL-6, Stem Cell Factor (SCF), thrombopoietin(TPO), FL3, erythropoietin(EPO), and GM-CSF are used independently or as a “cocktail type” mixed with each other according to the cell type, culture and differentiation level. So, researchers can achieve media optimization through our mixed vessel above instrument, accordingly optimizing the goal of the cell culture and differentiation research.
Complex media can be prepared initially in a separate large volume mixed vessel outside of the bio safety chamber, which can be supplied to a secondary small volume mixed vessel inside of the bio safety chamber with a fixed interval and volume. The secondary mixed vessel is located inside of the bio safety chamber along with cell culture cartridge, under the same temperature environment. At the secondary Mixed vessel, researchers can supply continuously or periodically a minimum amount of cytokine, growth factor, and nutrient to the cartridge as intended through micromini peristaltic pumps for the purpose of culture and differentiation research. As the peristaltic pumps related to the initial mixed vessel are the big ones, and the pumps used for the secondary mixed vessel are the micro peristaltic pumps located in the bio safety chamber, the amounts of supply can vary. Some researchers might prefer to put a small mixing chamber inside of the cell culture cartridge instead of using a separate secondary mixed vessel, and mix the complex media from the first mixed vessel and cytokine, growth factor, and others from micro peristaltic pumps, and then supply it to the cell. Biotron’s bioreactor can perform this type of media preparation without the threat of contamination. It can also minimize the time and cost for media optimization and be free from contamination during the culture. Also, it can perform media optimization study by not only using one cell culture cartridge but also using a multitude of cell culture cartridges by supplying different types of formulated media respectively. Cell culture in TotiCell, PluriCell, and MultiCell bioreactor
The essential conditions for stem cells that should be taken into consideration are adherence, suspension, and initial cell inoculation density as well as other factors for general animal cells such as media, nutrient, pH 7.4 in general, 37℃ temperature, dissolved oxygen, CO2 or O2 partial pressure, antibiotics for the prevention of contamination, growth factor, hormone. In addition, for stem cell culture, it is very important to have an advanced consensus with the aim cell after differentiation, and to consider the influence of the conditioned media to the final cell and cell to cell interaction through feeder cell. Physical stimuli that fit with the final differentiation induced cell environment and solid-space adaptation are also very important to maximize the treatment effect through easy adaptation to the new environment and resumption of differentiation after transplantation.
Considering that the culture scale for stem cells is generally lesser than 10ml, it is impossible for the pre existing animal cell culture bioreactors to achieve that. However, Bioitron‘s bioreactor can culture cells not only smaller than 10 ml in scale, but also lesser than 1 ml in scale by using disposable cartridges. A variety of other cell culture methods can also be performed depending on the variety of cartridge composition.
Even though it is a very small scale, the Biotron-developed “all-in-one” sensor can utilize ISFET(Ionsensitive FET) that can measure temperature, pH, and DO simultaneously into the nearest position to the cell where a measurement of the cell culture conditions can be made in real time, so, it can actively control every necessary condition based on the measured data.
It can also support three (3) types of images, such as optical images, confocal images, and hologram images. In addition users have the choice to select the ideal one considering the prices and characteristics of the live cell image devices. If images are transmitted to a computer, the image analysis software in the computer can perform researcher-specified patterns based on specific cell counting as well as general cell counting. In addition, it provides the solution that can trace and analyze the change that occurs during of live cell imagery, cell number and tracking including chemotaxis in accordance with manipulation during cell culture. Cell culture vessels are provided for suspension cells and cell culture cartridges for adherent cells. Each having a variety types of vessels or cartridges designed to fit various types of cell characteristics. Our policy is to provide users with optimal customized cell culture and cell culture cartridges according to the customers’special requests.
Cell culture vessels and cell culture cartridges have a separate independent temperature control device, which can perform a precise temperature control. Also, the temperature of the inside of the bio safety chamber can be controlled accurately, assisting in the direct control of the temperature in the cell culture vessel and cell culture cartridge, thus providing the best environment for maintaining precise temperature. As there are 4 gas supplying ports for O2,CO2,N2, and air inside the bio safety chamber, it can directly infuse gas quantities into the cell culture cartridge by specific amounts, for example, 0.01 ~ 21% O2, or 0 ~ 10% CO2, in explicit intervals, and also can install an independent mixed vessel in the front end and dissolve several gases, and then infuse them one at a time.
Also, some researchers want to provide the cultured cell with outside O2, CO2 gases by mounting a gas permeable membrane on the cell culture cartridge. For this purpose, the bio safety chamber inside has the ability to supply O2 and CO2 gases and maintain a suitable level.
The small and sophisticated bio safety chamber that is able to completely prevent contamination from outside can be adjusted for biohazard cleanliness levels by users from class 100 to class 100,000 for the necessary aseptic work area, and we are well prepared for that with a variety of HEPA filters and circulation fans .For the optimized culture of cells used for stem cell therapy as well as cell therapy, a variety types of cell culture cartridges that fit with the culture characteristic of each cell have been prepared. Cell culture cartridges are mainly designed for adherent cells, whereas, suspension cells are mainly used in glass vessels.
We provide customers with optimal customized cell culture cartridges taking into consideration the type and amount of supplying substances that are suitable for the cell to be cultured, such as cell culture capacity, cytokine, and nutrient, membrane type and structural arrangement, as well as type and optimal capacity of gas to be supplied to the cell.
Although it is the same cell, the design might be different depending on the research purpose of mass culture and differentiation of study. For example, even if it is the same cell, the results may be different between the suspension culture and adherent cell culture. As there are various types of material and structure in micro carrier or scaffold, soft agar gel, the culture results can be different every time. Furthermore if vital stimuli are applied, the results can also vary, so the bioreactor design should be made to fit each individual research need. However, there has been no bioreactor that could satisfy the variety of conditions of researchers‘ needs. Therefore cell therapy research can not help staying at this limited boundary. The cell therapy research through those varieties of bioreactor comparison research can produce momentous results in both academic and commercial areas.
Our policy is to provide researchers with optimal designed cartridges and vessels in accordance with whatever kinds of requests the researcher proposes.
For stem cell media, generally like wise conventional animal cell culture, some suitable amount of FBS(or, serum-free) and antibiotics are added to basal medium, such as DMEM, IMDM, MEMa, and RPMI 1640.
For example, for hematopoietic stem cell culture, several cytokines such as IL-3, IL-6, Stem Cell Factor (SCF), thrombopoietin(TPO), FL3, erythropoietin(EPO), and GM-CSF are used independently or as a “cocktail type” mixed with each other according to the cell type, culture and differentiation level. So, researchers can achieve media optimization through our mixed vessel above instrument, accordingly optimizing the goal of the cell culture and differentiation research.
Complex media can be prepared initially in a separate large volume mixed vessel outside of the bio safety chamber, which can be supplied to a secondary small volume mixed vessel inside of the bio safety chamber with a fixed interval and volume. The secondary mixed vessel is located inside of the bio safety chamber along with cell culture cartridge, under the same temperature environment. At the secondary Mixed vessel, researchers can supply continuously or periodically a minimum amount of cytokine, growth factor, and nutrient to the cartridge as intended through micromini peristaltic pumps for the purpose of culture and differentiation research. As the peristaltic pumps related to the initial mixed vessel are the big ones, and the pumps used for the secondary mixed vessel are the micro peristaltic pumps located in the bio safety chamber, the amounts of supply can vary. Some researchers might prefer to put a small mixing chamber inside of the cell culture cartridge instead of using a separate secondary mixed vessel, and mix the complex media from the first mixed vessel and cytokine, growth factor, and others from micro peristaltic pumps, and then supply it to the cell. Biotron’s bioreactor can perform this type of media preparation without the threat of contamination. It can also minimize the time and cost for media optimization and be free from contamination during the culture. Also, it can perform media optimization study by not only using one cell culture cartridge but also using a multitude of cell culture cartridges by supplying different types of formulated media respectively. Cell culture in TotiCell, PluriCell, and MultiCell bioreactor
The essential conditions for stem cells that should be taken into consideration are adherence, suspension, and initial cell inoculation density as well as other factors for general animal cells such as media, nutrient, pH 7.4 in general, 37℃ temperature, dissolved oxygen, CO2 or O2 partial pressure, antibiotics for the prevention of contamination, growth factor, hormone. In addition, for stem cell culture, it is very important to have an advanced consensus with the aim cell after differentiation, and to consider the influence of the conditioned media to the final cell and cell to cell interaction through feeder cell. Physical stimuli that fit with the final differentiation induced cell environment and solid-space adaptation are also very important to maximize the treatment effect through easy adaptation to the new environment and resumption of differentiation after transplantation.
Considering that the culture scale for stem cells is generally lesser than 10ml, it is impossible for the pre existing animal cell culture bioreactors to achieve that. However, Bioitron‘s bioreactor can culture cells not only smaller than 10 ml in scale, but also lesser than 1 ml in scale by using disposable cartridges. A variety of other cell culture methods can also be performed depending on the variety of cartridge composition.
Even though it is a very small scale, the Biotron-developed “all-in-one” sensor can utilize ISFET(Ionsensitive FET) that can measure temperature, pH, and DO simultaneously into the nearest position to the cell where a measurement of the cell culture conditions can be made in real time, so, it can actively control every necessary condition based on the measured data.
